IGF1-LR3 1mg

• 🧪

  Reconstitution

  IGF1-LR3 is supplied as a 1 mg lyophilised vial. Reconstitution practice in cell-culture work typically uses 1 mL of bacteriostatic water or 0.1 % BSA-acidic-buffer to yield a 1 mg/mL stock; further dilution into culture medium follows the assay protocol. The Long R3 variant adsorbs to glass and untreated polystyrene more readily than other peptides — low-binding polypropylene is the conventional choice.

• 📊

  Published protocols

  Cell-culture protocols typically use IGF1-LR3 at 1–100 ng/mL in serum-free or low-serum media for proliferation, protein-synthesis, or survival endpoints (Ballard/Francis CSIRO work; subsequent muscle-cell and adipocyte studies). Preclinical rodent anabolic-model dosing in the cited Tomas 1992 work used roughly 100–250 µg/kg/day subcutaneous infusion. There is no published research-protocol standard equivalent to a clinical dose.

• ⏱

  Half-life

  Native IGF-1 plasma half-life is ~10 minutes free / ~12–15 hours bound to IGFBP-3 ternary complex. IGF1-LR3's reduced IGFBP binding produces a longer free-fraction in-vitro effective half-life (commonly reported ~20–30 hours in cell-culture media); circulating-plasma half-life in vivo is also extended relative to native IGF-1 in the cited Tomas 1992 rodent work, though exact pharmacokinetic figures vary by model.

• 🧊

  Post-reconstitution stability

  Reconstituted IGF1-LR3 retains activity for ~14–28 days at 2–8 °C protected from light when stored in low-binding polypropylene. Repeated freeze-thaw degrades activity; aliquoting before freezing is conventional. Activity loss is gradual rather than abrupt.

Anabolic-dose range from Tomas et al. (Biochem J 1992, PMID 1540680). Variant characterisation from King et al. (J Mol Endocrinol 1992, PMID 1547202) and Francis et al. (Biochem J 1992, PMID 1280101). IGF1R pharmacology summarised in Adams et al. (Cell Mol Life Sci 2000, PMID 10961344). IGFBP binding modulation in Hwa et al. (Endocr Rev 1999, PMID 10605625). PMIDs and dose figures should be cross-checked against PubMed before citation in any publication.