New England Journal of Medicine
Once-Weekly Semaglutide in Adults with Overweight or Obesity (STEP 1)
Wilding JPH, Batterham RL, Calanna S, et al.
2021 · DOI: 10.1056/NEJMoa2032183 · PMID: 33567185
Read on PubMed ↗
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Metabolics & Appetite
Semaglutide is a 31-amino-acid analogue of human glucagon-like peptide-1 (GLP-1) bearing a fatty-acid (C18 di-acid) side chain that confers albumin binding and an extended circulating half-life. A substantial published literature — spanning the STEP, SUSTAIN, PIONEER, and SELECT phase 3 trial programmes — has characterised how cohorts dosed with semaglutide engage the GLP-1 receptor across glycaemic, body-weight, and cardiovascular endpoints. Reported outcomes in these clinical research cohorts are not predictive of any individual response and do not constitute an endorsement of efficacy or safety outside controlled trial conditions. For laboratory and research use only.
Research focus
Summary of contexts in which this compound appears in the preclinical literature. See cited studies for primary sources.
Semaglutide is a 31-amino-acid analogue of human glucagon-like peptide-1 (GLP-1) bearing a fatty-acid (C18 di-acid) side chain that confers albumin binding and an extended circulating half-life. A substantial published literature — spanning the STEP, SUSTAIN, PIONEER, and SELECT phase 3 trial programmes — has characterised how cohorts dosed with semaglutide engage the GLP-1 receptor across glycaemic, body-weight, and cardiovascular endpoints. Reported outcomes in these clinical research cohorts are not predictive of any individual response and do not constitute an endorsement of efficacy or safety outside controlled trial conditions. For laboratory and research use only.
Areas of research interest
Technical specifications
Compound Information
Long-acting GLP-1 receptor agonist (31 amino acids)
Storage & Stability
Lyophilized (powder)
-20°C • long-term stable in sealed lyophilized form
Reconstituted
2-8°C • use within 30 days of reconstitution
Light
Protect from light during storage and reconstitution
Room temperature
Short-term tolerant during shipping; minimise extended exposure above 25°C
Compound-specific notes
Albumin-binding fatty-acid moiety is sensitive to oxidation; minimise headspace air contact in reconstituted vials and avoid repeated freeze-thaw cycles.
Dosing in the literature
Observational summary of values reported in published research. Not a guide for human or animal use.
Reconstitution
Typically 1–2 mL bacteriostatic water per 10 mg vial, yielding ~5–10 mg/mL working concentration in research preparations.
Published protocols
STEP phase 3 obesity trials evaluated weekly SC doses titrated 0.25 → 2.4 mg over 16 weeks. SUSTAIN diabetes trials used 0.5 and 1.0 mg weekly. SELECT cardiovascular outcome trial used 2.4 mg weekly.
Half-life
~7 days in human pharmacokinetic studies, extended by C18 fatty-acid albumin binding.
Post-reconstitution stability
~28 days at 2–8 °C in BAC-water solution.
Values from cited STEP, SUSTAIN, SELECT, and PIONEER phase 3 trial publications. Clinical-trial dose ranges only — no general-use protocols established.
Sources & references
New England Journal of Medicine
Once-Weekly Semaglutide in Adults with Overweight or Obesity (STEP 1)
Wilding JPH, Batterham RL, Calanna S, et al.
2021 · DOI: 10.1056/NEJMoa2032183 · PMID: 33567185
Read on PubMed ↗The Lancet
Semaglutide 2·4 mg once a week in adults with overweight or obesity, and type 2 diabetes (STEP 2)
Davies M, Færch L, Jeppesen OK, et al.
2021 · DOI: 10.1016/S0140-6736(21)00213-0 · PMID: 33667417
Read on PubMed ↗JAMA
Effect of Subcutaneous Semaglutide vs Placebo as an Adjunct to Intensive Behavioral Therapy on Body Weight (STEP 3)
Wadden TA, Bailey TS, Billings LK, et al.
2021 · DOI: 10.1001/jama.2021.1831 · PMID: 33625476
Read on PubMed ↗JAMA
Effect of Continued Weekly Subcutaneous Semaglutide vs Placebo on Weight Loss Maintenance (STEP 4)
Rubino D, Abrahamsson N, Davies M, et al.
2021 · DOI: 10.1001/jama.2021.3224 · PMID: 33755728
Read on PubMed ↗JAMA
Effect of Weekly Subcutaneous Semaglutide vs Daily Liraglutide on Body Weight (STEP 8)
Rubino DM, Greenway FL, Khalid U, et al.
2022 · DOI: 10.1001/jama.2021.23619 · PMID: 35015037
Read on PubMed ↗New England Journal of Medicine
Once-Weekly Semaglutide in Adolescents with Obesity (STEP TEENS)
Weghuber D, Barrett T, Barrientos-Pérez M, et al.
2022 · DOI: 10.1056/NEJMoa2208601 · PMID: 36322838
Read on PubMed ↗New England Journal of Medicine
Semaglutide and Cardiovascular Outcomes in Patients with Type 2 Diabetes (SUSTAIN-6)
Marso SP, Bain SC, Consoli A, et al.
2016 · DOI: 10.1056/NEJMoa1607141 · PMID: 27633186
Read on PubMed ↗New England Journal of Medicine
Semaglutide and Cardiovascular Outcomes in Obesity without Diabetes (SELECT)
Lincoff AM, Brown-Frandsen K, Colhoun HM, et al.
2023 · DOI: 10.1056/NEJMoa2307563 · PMID: 37952131
Read on PubMed ↗The Lancet
Oral semaglutide versus subcutaneous liraglutide and placebo in type 2 diabetes (PIONEER 4)
Pratley R, Amod A, Hoff ST, et al.
2019 · DOI: 10.1016/S0140-6736(19)31271-1 · PMID: 31186120
Read on PubMed ↗The Lancet Diabetes & Endocrinology
Efficacy and safety of once-weekly semaglutide versus exenatide ER in subjects with type 2 diabetes (SUSTAIN 3)
Ahmann AJ, Capehorn M, Charpentier G, et al.
2017 · DOI: 10.2337/dc17-0417 · PMID: 29074815
Read on PubMed ↗10 peer-reviewed references listed. Citations verified against NCBI PubMed records. For research and educational reference only — does not constitute medical advice.
Researcher FAQ
No. Aero Peptides materials are sold exclusively for in-vitro research and laboratory analytical use. They are not intended to diagnose, treat, cure, or prevent any disease, and are not approved for human or animal administration.
By placing an order, you confirm that you are a qualified researcher and that the material will be used in accordance with all applicable institutional and jurisdictional regulations.
Each lot is independently tested for identity, purity, and mass using HPLC and mass spectrometry. The COA lists the lot number, test date, assay results, and the laboratory that performed the analysis.
Public COA documents are being prepared. In the meantime, you can request a COA for your specific lot and we’ll email it within 1 business day.
Orders ship quickly after your order is placed, from the US. Lyophilized peptides are shipped with insulation appropriate to season; short-term ambient excursions during transit do not measurably affect potency.
On receipt, store at −20°C in the sealed vial until reconstitution. See the handling matrix for full storage parameters. Tracked shipping is included; we ship to all US states and most international destinations.
Most peptides are reconstituted with bacteriostatic water (BAC). The volume depends on the target working concentration. Use our Peptide Calculator to compute volume, U-100 insulin syringe units, and doses per vial from peptide mass and target dose.
Once reconstituted, store at 2–8°C and use within 30 days. Protect the vial from light during storage and handling.
Because peptides are temperature-sensitive research materials, we cannot accept returns of opened or unsealed vials. If a shipment arrives damaged or the seal is broken on arrival, contact us within 7 days and we’ll replace or refund.
See our full returns policy for details.
Don’t see your question? Contact our research team — we typically reply within one business day.
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⚠ Important research notice
Not for human consumption. This product is sold exclusively for research and educational purposes. It is not intended to diagnose, treat, cure, or prevent any disease.
All clinical trial data and research findings presented on this page are sourced from peer-reviewed journals and official publications. They are provided for educational reference only and should not be interpreted as medical advice or product claims.
By purchasing this product, you confirm that you are a qualified researcher and will use it in accordance with all applicable laws and regulations.